Unclassified Nsn 7540 - 01 - 280 - 5500

Rapid characterization of biological specimens is increasingly important in research and clinical applications. While current optical and chemical detection techniques can effectively analyze biological systems, a number of disadvantages restrict their versatility. As examples: most samples require advanced processing such as PCR amplification or chemical treatment, and photobleaching often limits the optically probing of fluorophore-tagged samples. Purely electronic techniques, such as those we propose here, provide solutions to many such problems, as they can probe a sample directly without requiring special modification. Equally important, readout is direct and, consequently, sensors can be easily integrated with electronics, making the entire system compact and robust. We developed a series of integrated microfluidics devices that are capable of detecting DNA in cells and the cell-surface expression of proteins on the cell-surface walls. As well, we developed an integrated microfludiics chip that is capable of performing simultaneous measurement of several sensors. (a) Papers published in peer-reviewed journals (N/A for none) A. Carbonaro and L. L. Sohn, A Resistive Pulse Sensor for Multianalyte Detection, Lab Chip 5, 1155-1160 (2005). List of papers submitted or published that acknowledge ARO support during this reporting period. List the papers, including journal references, in the following categories: (b) Papers published in non-peer-reviewed journals or in conference proceedings (N/A for none) 1. S. W. Mohanty, L. L. Sohn, and D. J. Beebe, Hybrid Polymer/Thin-Film Impedance System for Label-Free Monitoring of Cells, 26th Annual International Conference, IEEE Engineering in Medicine and Biology Society, 2004. 2. O. A. Saleh and L. L. Sohn, Biological Sensing with an On-Chip Resistive Pulse Analyzer, 26th Annual International Conference, IEEE Engineering in Medicine and Biology Society, 2004. 3. I. H. Chan, A. Carbonaro, and L. L. Sohn, Artificial Pores for Performing Immunoassays, to appear in the International Conference on Miniaturized Chemical and Biochemical Analysis Systems 2004, Kluwer Academic Publishers (2004). 1.00 Number of Papers published in peer-reviewed journals: Number of Papers published in non peer-reviewed journals: (c) Papers presented at meetings, but not published in conference proceedings (N/A for none) 3.00 Number of Papers not Published: (d) Manuscripts 0.00 Number of Manuscripts: 0.00 Number of Inventions: